1.    Nelson, W. J., Vorgias, C. E. and Traub, P. (1982). A Rapid Method for the Large Scale Purification of the Intermediate Filament Protein Vimentin by Single-Stranded DNA Cellulose Affinity Chromatography. Biochem. Biophys. Res. Commun. 106, 1141-1147.

2.    Traub, P., Nelson,W. J., Kühn, S. and Vorgias, C. E. (1983). The Interaction in vitro of the Intermediate Filament Protein Vimentin with Naturally Occurring RNAs and DNAs. J. Biol. Chem. 258, 1456-1466.

3.    Vorgias, C. E. and Traub, P. (1983). Isolation of Glial Fibrillary Acidic Protein from Bovine Brain White Matter and its Purificatioon by Affinity Chromatography on Single-Stranded DNA Cellulose. Biochem. Biophys. Res. Comm. 115, 68-75.

4.    Traub, P. and Vorgias, C. E. (1983). Involvement of the N-Terminal Polypeptide of Vimentin in the Formation of Intermediate Filaments. J. Cell Sci. 63, 43-67.

5.    Vorgias, C. E. and Traub, P.(1983).Isolation, Purification and Characterization of the Intermediate Filament Protein Desmin from Porcine Smooth Muscle. Prep. Biochem. 13, 227-243.

6.    Traub, P. and Vorgias, C. E. (1984). Differential Effect of Arginine Modification with 1,2-Cyclohexanedione on the Capacity of Vimentin and Desmin to Assemble into Intermediate Filaments and to Bind to Nucleic Acids. J. Cell Sci. 65, 1-20.

7.    Traub, P., Vorgias, C. E. and Nelson, W. J. (1985). Interaction in vitro of the Neurofilament Triplet Proteins from Spinal Cord with Natural RNAs and DNAs. Mol. Biol. Rep. 10, 129-136.

8.    Vorgias, C. E. and Traub, P. (1986). Nucleic Acid-Binding Activities of the Intermediate Filament Subunits Desmin and Glial Fibrillary Acidic Protein. Z. Naturforsch. 416, 897-909.

9.    Vorgias, C. E. and Traub, P. (1986). Efficient Degradation in vitro of all Intermediate Filament Subunits Proteins by the Ca2+-Activated Neutral Thiol Proteinase from Ehrlich Ascite Tumor Cells and Procine Kidney. Bioscience Reports, 6, 57-64.

10.    Zimmermann, H.-P., Plagens, U., Vorgias, C. E. and Traub, P. (1986). Changes in the Organization of Non-Epithelial Intermediate Filaments Induced by Triethyl Lead Chloride. Exp. Cell Res., 167, 360-368.

11.    Kuehn, S., Vorgias, C. E. and Traub, P.(1987). Interaction in vitro of Non-Epithelial Intermediate Proteins with Supercoiled Plasmid DNA J. Cell Sci. 87, 543-554.

12.    Vorgias, C. E., Peridis, G. A., Traub, P. and Sekeris, C. E. (1988). Colchicine, Colcemide and Cytochalasin B do not Affect Translocation of the Glucocorticoid  Hormone-Receptor in Rat Thymocytes or Ehrlich Ascites Cells. Bioscience Reports, 8, 193-197.

13.    Gallwitz, D., Haubruck, H., Molenaar, C., Prange, R., Putzicha, M., Schmitt, H.-D., Vorgias,C. E. and Wagner, P. (1988). Structural and Functional Analysis of ypt Proteins, a Family of ras-Related Nucleotide-Binding Proteins in Eucaryotic Cells. (1989) In: The Guanine - Nucleotide Binding Proteins, Common Structural and Functional Properties, Edited by L. Bosch, B. Kraal and A. Parmeggiani, NATO ASI Series, Life Sciences Vol. 165, 257-264.

14.    Haubruck, H., Prange, R., Vorgias, C. E. and Gallwitz, D. (1989). The ras-related mouse ypt1 protein can functionally replace the YPT1 gene product in yeast EMBO J., 8, 1427-1432.

15.    Wilson, K. S., Vorgias, C. E., Tanaka, I., White, W. S. and Kimura, M. (1990). The Thermostability of DNA Binding Protein HU from Thermophilic and Mesophilic Bacilli . Protein Engineering 4,11-22.

16.    Vorgias, C. E., Kingswell, A. J., Dauter, Z., Wilson, K. S. (1991). Cloning, overexpression, purification and crystallisation of ribosomal protein L9 from Bacillus stearothermophilus. FEBS lett. 286, 204-208.

17.    Vorgias, C. E., Lemaire, H-G. and Wilson, K. S. (1991). Overexpression and Purification of the Galactose Operon Enzymes from E. coli.Protein Overexpression and Purification 2, 330-338.

18.    Vorgias, C. E. and Wilson, K. S. (1991). A Rapid Method for the Purification of Recombinant Integration Host Factor Protein Overexpression and Purification 2, 317-320.

19.    Padas, M. P., Wilson, K. S. and Vorgias, C. E. (1992). DNA Binding Protein from Mesophilic and Thermophilic Bacilli: Cloning, Overexpression and Purification. Gene 117, 39-44.

20.    Vorgias, C. E., Kingswell, A. J., Dauter, Z., Oppenheim, B. A. (1992) Crystallisation of Recombinant Chitinase from the cloned chiA gene of Serratia marcescents. J. Mol. Biol. 226, 897-898.

21.    Tews, I., Dauter, Z. and Oppenheim, B. A and Vorgias, C. E. (1992) Crystallisation of Recombinant Chitobiase from Serratia marcescents.  J. Mol. Biol. 228, 696-697.

22.    Sayers, Z., Brouillon, P., Vorgias, C. E. Nolting, H. F., Hermes, C. and Koch, M. H. J. (1993). Cloning and expression of Saccharomyces cerevisiae copper-metallothionein gene in Escherichia coli and characterization of the recombinant protein Eur. J. Biochem. 212, 521-528

23.    Vorgias, C. E., Tews, I., Perrakis, A. Wilson, K. S. and Oppenheim, B. A (1993)Purification and characterisation of the recombinant chitin degrading enzymes Chitinase and Chitobiase from Serratia marcescents. In Chitin Enzymology (Muzzarelli, R. A. A. ed.) pp 417-422.

24.    Perrakis, A., Wilson, K. S., Chet. I, Oppenheim, A. O. and Vorgias, C. E. (1993)Phylogenetic relatioships of chitinases. In Chitin Enzymology (Muzzarelli, R. A. A. ed.) pp 217-232.

25.    Rypniewski, W. R.,  Perrakis, A, Vorgias, C. E. and Wilson, K. S (1994) Evolutionary divergence and conservation of trypsins. Protein Engineering 7, 57-64.

26.    Vis, H., Boelens, R., Mariani, M., Stroop, R.,Vorgias, C. E., Wilson, K. S. and Kaptein, R. (1994) 1H, 13C and 15N resonance assignments and secondary strucutre analysis of the HU protein from Bacillus stearothermophilus using two- and three-dimensional double- and triple-resonance heteronuclear magnetic resonance spectroscopy. Biochemistry 33, 14858-14870

27.    Perrakis, A., Tews, I., Dauter, Z., Wilson, K.S. and Vorgias, C. E. (1994) X-ray structure analysis of Chitinase A from Serratia marcescens. In Chitin World (eds Karnicki, Z.S., Wojtasz-Pajak, A., Brzeski, M.M. and Bykowski, P.J.) pp 408-415.

28.    Tews, I, Vincentelli, R., Perrakis, A., Dauter, Z., Wilson, K.S. and Vorgias, C. E. (1994) X-ray structure analysis of Chitobiase from Serratia marcescens. In Chitin World (eds Karnicki, Z.S., Wojtasz-Pajak, A., Brzeski, M.M. and Bykowski, P.J.) pp 415-423.

29.    Perrakis, A., Tews, I., Dauter, Z., Oppenheim, A., Chet, I.,Wilson, K. S. and Vorgias, C. E. (1994) Crystal structure of a bacterial chitinase at 2.3 Å resolution Structure 2, 1169-1180.

30.    Vis, H., Mariani, M., Vorgias, C. E., Wilson, K. S. and Kaptein, R. and Boelens, R. (1995) Solution strucutre of the HU Protein from Bacillus stearothermophilus. J. Mol. Biol. 254, 692-703.

31.    Sitrit, Y., Vorgias, C.E., Chet, I. and Oppenheim, A. O. (1995) Cloning and primary structure of a chiA gene from Aeromonas caviae J. Bacteriology 177, 4187-4189.

32.    Sikorski, M. M., Topunov, A. F., Strozycki, P. M., Vorgias, C. E., Wilson, K. S. and Legoski, A. B. (1995) Cloning and expression of plant leghrmoglobin cDNA of Lupinus luteus in Escherichia coli and purification of the recombinant protein. Plant Science 108, 109-117.

33.    Boelens R, Vis H, Vorgias Ce, Et Al. Structure And Dynamics Of The DNA Binding Protein Hu From Bacillus Stearothermophilus By NMR Spectroscopy Biopolymers 40 (5): 553-559 1996

34.    Tews, I., Perrakis, A., Dauter, Z, Oppenheim, A., Wilson, K. S.and Vorgias, C. E. (1996) Bacterial chitobiase structure provides insight into catalytic mechanism and the basis of Tay-Sachs disease. Nature Structural Biology 3, 638-648

35.    Vis, H., Vageli, O., Nagel, J., Vorgias, C. E., Wilson, K. S. and Kaptein, R. and Boelens, R. (1996) NMR study of the interaction of the HU Protein from Bacillus stearothermophilus with DNA. Magnetic Resonance in Chemistry. 34, 81-86.

36.    Tews, I., Vincentelli, R. and Vorgias, C. E. (1996) N-acetylglucosaminidase (chitobiase) from Serratia marcescens: gene sequence, and protein production and purification in Escherichia coli. Gene 170, 63-67.

37.    Tews, I., Wilson, K. S. and Vorgias, C. E. (1996) Enzymatic mechanism of N-acetylglucosaminidase revealed by structural studies on enzyme substrate-inhibitor complexes. Advances in Chitin Science Vol. 1. pp 26-33

38.    Perrakis, A., Ouzounis, C. Wilson, K. S. and Vorgias, C. E. (1996) Implications of the 3-D structure determination of family 18 chitinases. Similarities with FnIII domains, oviductal proteins and narbonins. Advances in Chitin Science Vol. 1. pp 34-41

39.    Kanellopoulos, P. N., Pavlou, K., Perrakis, A., Agianian, B., Vorgias, C. E., Mavrommatis, C., Soufi, M., Tucker, P. A. and Hamodrakas, S. J. (1996) The crystal structure of the complexes of Concanavalin A with 4'-nitrophenyl - a - D - mannopyranoside and 4'- nitrophenyl - a - D -glucopyranoside. J. Structural Biology 116, 345-355.

40.    Vorgias C. E., Perrakis, A., Tews, I. (1996)  Structure function studies on the chitinolytic enzymes of Serratia marcescens chitinase and chitobiase. Annals of the New York Academy of Science. 799, 190-192.

41.    Perrakis, A., Tews I and Vorgias, C. E. (1996) Chitinases, Hevamine and Chitobiases: "Faraway and yet so closed" In Chitin Enzymology (Muzzarelli, R. A. A. ed.) Vol 2, pp 109-122.

42.    Tews I., Wilson, K.S and Vorgias, C. E. (1996) Structural studies on N-acetylglucosaminidase enzyme-inhibitor complexes In Chitin Enzymology (Muzzarelli, R. A. A. ed.) Vol 2, pp 213-225.

43.    Fernandes, M.J.G., Leclerc, D., Henrissat. B., Vorgias, C.E., Gravel, R., Hechtman, P., and F. Kalpan.  Identification of candidate active site residues in lysosomal b-hexosaminidase A. J. Biol. Chem. 272, 814-820

44.    Chernin, L. S., L.de la Fuente., Sobolev, V., Haran, S., Vorgias, C.E., Oppenheim, A.O. and Chet, I. (1997) Molecular Cloning, Structural analysis and Expression in Escherichia coli of a Chitinase Gene from Enterobacter agglomerans. Appl. Env. Microbiology 63, 834-839

45.    Spindler-Bath M., Blattner, R., C. E. Vorgias and Spindler, K-D. (1998)  Inhibition of two Family 18 Chitinases by various Allosamidin Derivatives Pesticides 52, 47-52

46.    Joergensen S., Vorgias, C. E. and Antranikian G. (1997) An exttracellular a-amylase from the Hypethermophilic Archeon Pyrococcus furiosus. Cloning, Sequencing, Characterization and Expression in E. coli and Bacillus subtilis. J. Biol. Chem. 272,16335-16342.

47.    Drouillard, S., Armand, S., Davies, G., Vorgias, C. E and Henrissat, B. (1997) Serratia marcescens chitobiase is a retaining glycosidase utilising substrate acetamido group participation Biochem. J. 328, 945-949

48.    Vorgias, C.E. (1997) Overproduction of the recombinant Chitinase A from Serratia marcescens in E. coli, fast purification, biochemical and biophysical characterisation. Chapter in Chitin Handbook (Muzzarelli and Peter  eds.) ISBN 88-86889-01-1.

49.    Vorgias, C.E. and Petratos, K. (1997) From the purified protein to the 3D-structure and enzymatic mechanism of Chitinase A from the chitinolytic soil bacterium Serratia marcescens. Chapter in Chitin Handbook Chapter in Chitin Handbook (Muzzarelli and Peter eds.) ISBN 88-86889-01-1.

50.    Christodoulou E and Vorgias, C. E (1998) Cloning, overproduction, purification and crystallisation of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima. Acta Crystal D54, 1043-1045.

51.    Vis H, Vorgias C.E, Wilson KS, Kaptein R, Boelens R (1998) Mobility of NH bonds in DNA-binding protein HU of Bacillus-stearothermophilus from reduced spectral density mapping analysis at multiple NMR fields. J. Biomolecular NMR 11, 265-277.

52.    Vorgias, C. E and Antranikian, G. (2000) Glycosyl Hydrolases from Extremophiles in Glycomicrobiology, pp313-339 (ed. by Doyle) Kluwer Academic/Plenum Publishers, N. York

53.    Boehlke K., Pisani F.M, Vorgias C.E., Frey B., Sobek H., Rossi M., and Antranikian G. (2000) PCR performance of the B-type DNA polymerase from the thermophilic euryarchaeon Thermococcus aggregans improved by mutations in the Y-GG/A Motif. Nucleic Acids Res. 2000 28: 3910-3917

54.    Prag, G., Papanikolau Y., Tavlas, G., Vorgias, C. E., Petratos, K. and Oppenheim A. O. (2000) Structures of chitobiase mutants complexed with the substrate Di-N-acetyl-D-glucosamine: the catalytic role of the conserved acidic pair, asparate 539 and clutamate 540. J. Mol. Biol. 300, 611-617.

55.    Fontaine T., Simenel C., Dubreucq G., Adam O., Delepierre M., Lemoine J., Vorgias C. E., Diaquin M., and Latgé J-P. (2000) Molecular Organization of the Alkali-insoluble Fraction of Aspergillus fumigatus Cell Wall J. Biol. Chem. 2000 275: 27594-27607.

56.    Lonhienne, TGA., Zoidakis, J, Vorgias, C. E, Bouriotis V. (2001) Purification, characterization and nucleotide sequence of a novel chitobiase from the Antarctic Arthrobacter sp. TAD20. J. Bacteriology 183, 1773-1779

57.    Lonhienne, TGA, J Zoidakis, Vorgias C. E, G Feller, C Gerday, V. Bouriotis (2001) Modular structure, local flexibility and cold-activity of a novel chitobiase from a psychrophilic antartic bacterium J. Mol. Biol. 310(2) 291-297

58.    E. Christodoulou, F. Duffner and C. E. Vorgias (2001) Overexpression, Purification and Characterization of a Thermostable Chitinase (Chi40) from Streptomyces thermoviolaceus OPC-520 Protein Expression and Purification 23, 97-105

59.    Y. Papanikolau, G. Prag, G Tavlas, C E. Vorgias┴, A. B. Oppenheim, K. Petratos (2001) Crystallographic Evidence for a Novel ‘Flip-Flop’ Mechanism for Chitinase A. Biochemistry 40(38) 11338-11343.

60.    Prog G., Vorgias, C. E. and Oppenheim, A-O (2001) Conservation and structural elements and catalytic mechanism in Serratia marcescens chitinolytic enzymes. Chitin Enzymology, 2001.Ancona

61.    Christodoulou E and Vorgias, C. E. (2002) The thermostability of the DNA binding protein HU from mesophilic, thermophilic and extreme thermophilic bacteria Extremophiles 6,21-31.

62.    Raves, M. L., Doreleijers, J., Vis, H., Vorgias, C. E., Wilson, K. S. Kaptein, R. (2001) Joint refinement as a tool for thorough comparison between NMR and X-ray data and structures of HU protein. J. Biomolecular NMR 21, 235-248

63.    Christodoulou E, Rypniewski, W. and Vorgias, C. E (2002) X-ray structure of the DNA binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima at 1.5 Å. Extremophilies. (submitted)

64.    Y. Papanikolau, C E. Vorgias, K. Petratos (2002) New purification and crystallization yield high-resolution structures of chitinase A and its complex with the inhibitor allosamidin. Acta D. (submitted)

65.    Christodoulou E., and Vorgias, C. E. (2002) Understanding heterologous protein overproduction under the T7 promoter: a practical exercise. Biochemical and Mol. Biol. Education (in press)